Equipment

The DaMBIC is equipped for imaging of samples ranging from single molecules and cells to tissue and small animals. Imaging modalities include:

  • General widefield fluorescence microscopy with Nikon Ti or Ti-2 Eclipse using Nikon NIS Elements
  • Confocal microscopy on one of the 2 following microscopes: Nikon A1 and Leica SP8
  • STED (STimulated Emission Depletion microscopy)
  • SIM (Structued Illumination Microscopy)
  • STORM (STochastic Optical Reconstruction Microscopy)
  • CARS (Coherent anti-Stokes Raman microscopy)
  • Spinning disk microscopy (fast image acquisition for live cell imaging)
  • TIRF (Total Internal Reflection Fluorescence microscopy)
  • FLIM (Fluorescence Lifetime IMaging)
  • FCS (Fluorescence Correlation Spectroscopy)
  • Two-photon microscopy
  • RICS  (Raster Imaging Correlation Spectroscopy)
  • AFM (Atomic Force Microscopy)
  • Ellipsometry
  • IVIS Spectrum (SUN)
  • PET CT (SUN) (Positron Emission Tomography, Computed Tomography)

Which microscope to choose:

For fast, straightforward imaging you probably need a widefield system.
Confocal and spinning disk, are ideal to image samples that are thicker than 10 um.
For very thick samples, multiphoton microscopy may be needed.
TIRF systems are used to image the area of the cell close to the coverslip.
If you need to resolve structures smaller than 200 nm, you will need super resolution like STED, SIM or STORM.
- But please contact us to discuss your application or scientific aim and we can find the most optimal technique together.